An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR.

An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR.

An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study,...

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Bibliographic Details
Main Authors: PEREIRA, W. J., BASSINELLO, P. Z., BRONDANI, C., VIANELLO, R. P.
Other Authors: WENDELL JACINTO PEREIRA, UFG; PRISCILA ZACZUK BASSINELLO, CNPAF; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Format: Artigo de periódico
Language:English
eng
Published: 2017
Subjects:
Gene normalization
Molecular breeding
Grain RNA extraction
Feijão
Phaseolus vulgaris
Melhoramento
RNA
Beans
Gene expression.
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076282
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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076282

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